Team:TUDelft/27 August 2009
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27 August 2009
Saeed
The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.
lane | Part | Expected Plasmid Size | Status |
1 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | ||
2 | !Fr1n upstream | 1900 | ok, but not needed |
3 | !Fr1n downstream | 1900 | ok |
4 | destination plasmid | 3000 | ok |