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Team:KU Seoul/Notebook
From 2009.igem.org
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Contents |
Lab
Protocols (from elsewhere and FAQs)
- How do I test the transformation efficiency of my competent cells?
- Please see the protocol on how to [http://www.openwetware.org/wiki/TOP10_chemically_competent_cells make competent cells] for instructions on how to calculate transformation efficiency.
Discussion
Team building
From instructors
- 10.07.09 We had a review on our two ideas from Prof.Choi. He told us the ideas are creative and good but not fit for IGEM's concept. We need more feedback and information on our ideas. We need to get more familiar with IGEM's tools. Two additional ideas about Scromboid food poisoning and lactic acid degradation were referred at the end.
- 17.07.09 Till our next meeting, we should study how systemic biology works and understand related tools. Cumulate ideas. In other words, brainstorm consistently. Hyuk-jin will help with setting up experiments. Lab note opend.
- 24.07.09 We narrowed our ideas in to two. They are "Metal Detector" and "Bactaeria Compass." Think about what parts would be used and how you would build the circuits. Visit the former iGEM teams and study how they carried on their projects.
- 31.07.09 Find out the exact mechanism of metal detection. Look up the parts that are available on iGEM home. See how various metals can be detected by using E.coli.
- 07.08.09 We decided to detect 4 metals; Cd, Hg, Pb, and Ars. They are all critical to the health if ingested. Think about what the outcome would be after detection. Because there are 4 metals, you can bring out 16 different results by combining them. It seems that the most vialbe instrument would be light.
Brainstroming
Ideas
- Biosurfactants
- Keratinase
- Deodorant
- Perfume-producing bacteria
- Heat absorber
- Bio pencil
Should we focus on the result, or the procedure?
Decision making
From Cheolwon Choi, 4th of July
Hi everyone. We narrowed our ideas to two subjects, which are "Morse Code" and "Bio Sticker." If you would like to share any other ideas, you are more then welcome. Let's discuss about specific models for these two subjects at the upcoming meeting. Our next meeting is on 10th of July, 3 p.m. I recommend you think about at least one of the two subjects and how they should be developed. Tell me your schedules via mail so that I can fix our meeting time to the most suitable for all. I would like to share my experience at the iGEM workshop at Dongkyung about Wiki and other stuffs that you might b interested, so bring your notebooks. As I said before, prepare a specific model for the meeting. Try to document your ideas so they could be shared with others. A specific model should include these: 1. How will the chosen subject be used? 2. What are its limits? 3. What materials would be needed? 4. What former knowledge would be required to process the subject? 5. Why did you choose the subject? 6. What contributions can you make? 7. Etc. See you next Friday.
Korean stuffs
- [http://openwetware.org/wiki/IGEM:KU_Seoul/2009/Notebook/iGEM2009 Lab Notebook@Openwetware (νκΈ)]
