Uppsala-Sweden/3 September 2009

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Transformation from registry plates

Top 10 cells from rack 10 (-80C)

Thaw on ice for 30 minutes Re-suspend DNA in 15µl of dH20 Add 1µl of DNA to TOP10 µl stir gently Let sit for 30min (I just did for 10min)

Put on SOC @ RT for 60min (Just did this for 5min; not so good idea with kan or ch as selective antibiotic but amp works fine) Transfer to plate or liquid culture

Inoculation ON cultures

B0034-Yadh2 (pSB1AK3)

Measurement of RBS-Adh2Y construct

100 times dilution: Media:Plasmidprep_090904.txt