Team:Chiba/Notebook/Calendar/9 September 2009

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Chiba Labwork 09Sep09.jpg Chiba Labwork 09Sep09-2.jpg


0800 main culture 

    divided injection 12.5ml of the culture to 10 50ml centrifuging tubes 
    WASH 3 times
    Add 12.5ml new culture
  
    Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant

    Do same operation every 15min to 2nd~10th tubes

    after all, mix the supernatant and solid media at 1:1
    make 10 concentration varieties of solid media
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