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Team:KULeuven/9 September 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
- FACS-ing the liquid cultures that were put under blue light on 08/09 gave no significant results.
- Motherplate for was made
- liquid cultures for checking the ligX were made.
- LC with were miniprepped, nanodropped, restriction digested and put on gel. The signals were good. The vector, pSB1A2, was cut from the gel and gel extracted (10ng/µl). This was ligated with (LigY).
Nanodrop:
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| (1) | 141,8 | 1,94 | |
| (2) | 139,5 | 1,99 | |
| (3) | 177,5 | 1,95 | |
| (4) | 141 | 1,95 |
[edit] Vanillin Production
- Restriction digest on Terminator
- Growth on SAMS plates (woohoo!)
- Transferred 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate
- Gel electrophoresis of all the 8 EF restriction digests (2 per colony)
- Cut and purified EF1 and EF3
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| EF1 | 5,2 | 1,43 | |
| EF3 | 4,3 | 1,45 |
[edit] Vanillin Receptor
- fluid cultures were made of rpoA, X+K and the motherplate of virG so we could miniprep this tomorrow
- ligation of puc and A was electroporated
- new ligation of X+K was tried out
