Autoinduction assays

Glucose time delay

Aims

• Characterise the tunable time duration it takes before GFP expression (M2 activation)

Assay

• The cells will be grown until OD= 0.7.

• The RFP value will be monitered, although it is the GFP values that are more critical in this assay.

• OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].

• The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)

• The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)

See Glucose time delay for details

Protein production assays

Cellulase Assay

Encapsulation assays

Colanic Acid

Genomic deletion assays

Restriction Assay