Autoinduction assays

Glucose time delay

Aims

• Characterise the tunable time duration it takes before GFP expression (M2 activation)

Assay

• The cells will be grown until OD= 0.7.

• The RFP value will be monitered, although it is the GFP values that are more critical in this assay.

• OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].

• The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)

• The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)

See Glucose time delay for details

Protein production assays

Cellulase Assay
PAH Assay

Encapsulation assays

Colanic Acid

Aims

Colanic acid biosynthesis is both time consuming and metabolically expensive. If the E.ncapsulator is to be used in an industrial setting, then colanic acid mediated protection must be highly efficient. Protective efficiency can be defined as the percentage increase in fluorescence per µl of colanic acid produced (when compared to control cells). The following assay can be used to elucidate this parameter.

Assay

• Growth of cells (Assay preparation).

• Measurement of cell density.

• Measurement of packed cell volume (PCV).

• Measurement of the % change in GFP fluorescence following acid incubation.

Click here for Colanic Acid assay details

Genomic deletion assays

Restriction Assay