M1 Genetic Circuit

This is the genetic circuit that constitutes protein production (Module 1).

LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. Thus, our proteins of interest, PAH or cellulase will not be produced.

To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production (Module 1) is de-repressed. PAH or cellulase will be produced by the E.ncapsulator.