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Template:Team:KULeuven/7 September 2009/VanillinProduction
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Revision as of 16:08, 10 September 2009 by JochemDeen (Talk | contribs)
- Miniprep from the four colonies of EF ligation
- Accidentally used double volumes of elution buffer on EF4
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| EF 1 | 115,5 | 1,98 | 2,31 |
| EF 2 | 105,2 | 1,96 | 1,66 |
| EF 3 | 95,1 | 2,05 | 0,98 |
| EF 4 | 52,7 | 2,06 | 0,97 |
- Gel electrophoresis of restriction from Sam5 and Sam8 looked good
- Purified and ligated the restriction from Sam5 and Sam8 overnight
- Concentration for sam8 was barely enough for ligation...
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| Sam5 | 10,1 | 2,11 | 0,04 |
| Sam8 | 3,1 | 3,96 | 0,02 |
