Template:Team:KULeuven/10 September 2009/VanillinProduction

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  • Gel electrophoresis of TER restriction
    • The vector for the TER is Psb1AK3 and has 3100bp, with an insert of ±3200 bp
    • Gel extraction
part concentration 260/280 260/230
TER 14,2 1,97
  • Created a motherplate from EF1
  • Ligated EF restriction + TER restriction
  • Transferred the SAMS 1 and SAMS 2 colonies to liquid medium