Team:Chiba/Notebook/Calendar/2 September 2009
From 2009.igem.org
Revision as of 08:07, 15 September 2009 by Kaoru Yamamoto (Talk | contribs)
(Lab Work Start! |>3_September_2009)
- prior culture(exploratory experiment)
medium:liquid culture medium antibiotic:Amp
We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.
Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)