Introduction

This module consists of producing the drug protein of interest. In order to describe the function of the module, two models have been developed.

Model for protein drug production:

Based on the Genetic circuit, a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug.

• In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH)
• When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced.

Our goals

The modelling aims to provide an overview and better understanding of the M1 system’s function by:

• Characterizing the system.
• Modeling to account for several factors that may reduce/hinder the production of the protein drug such as:
  • Lac promoter leakiness
  • IPTG toxicity
  • Stability of output protein

This module is an integral part of the design, as large-scale commercialization of the drug of interest depends on finding the optimal conditions for protein production. This model has also helped the Wetlab to plan some of the experiments.

• The Model
  
• Simulations