Team:UNIPV-Pavia/Methods Materials/Transformation

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Protocols

Transformation

(estimated time: 3 hours and 30 min + 12-16 hours overnight incubation)

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Put 1-2 µl of DNA resuspension or ligation into thaw TOP10 cells tube (every tube contains approximately 60 µl of competent cells).
Incubate on ice for 30 min.
Heat shock: 42°C for 1 min.
Put transformed TOP10 tube on ice and then add 250 µl SOC medium.
Incubate 1 hour at 37°C, 220 rpm.
Plate 200 µl of the solution on a proper agar plate.
Incubate overnight at 37°C.

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