Template:Team:KULeuven/16 September 2009/VanillinProduction

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Revision as of 13:45, 19 September 2009 by JochemDeen (Talk | contribs)
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  • Restrictions from yesterday were put on gel (COMT2, 3, SAMS, Promotor)
    • SAMS and promotor( J23110??) were gel purified
part concentration 260/280 260/230
SAMS 8,5
Promotor 4,5
  • Ligation of SAMS + promotor
    • 28 μl of SAMS and 11 μl from promotor, almost 4:1 ratio (although maybe closer to 3:1)
  • Electroporation of EFT ligation from yesterday on Km plates (Ter vector has Km resistance, so a closing of EF vector on its own doesn't grow!)
  • Miniprep
part concentration 260/280 260/230
1 69,2 1,99
2 34,5 2,01
3 74,9 2,03
  • The liquid culture of SAMS was used for the glycerol stock and send for sequencing
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