"

 

From 2009.igem.org

Home The Project Motivation Solution Results & Conclusions References Materials & Methods Measurements Instruments Protocols Software Developed Parts Submitted Characterization The Team Notebook Week-book Freezer Management Biological Safety Pavia History University Gallery Sponsors Gallery Team Locations		Protocols
	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

DNA precipitation with sodium acetate

(estimated time: 1 hour)

Add 1/10 DNA solution volume of sodium acetate 3 M Add 2.5 DNA solution volume of absolute ethanol Freeze at -80°C for 30 min Centrifuge at 13000 rpm, 4°C for 20 min Decant supernatant Add 50 µl of 70% ethanol Centrifuge at 13000 rpm, 4°C for 20 min Remove all supernatant with a pipette Air dry pellet until ethanol is totally removed Elute with 5-10 µl of ddH2O

Sodium acetate Absolute ethanol Ethanol 70% ddH2O DNA sample

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers