Team:Imperial College London/Wetlab/Protocols/Autoinduction

| Secondary Carbon Source Diauxie Growth |

• To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling
• To determine the best secondary carbon source for optimal growth

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Glucose time delay

Aims

• Characterise the tunable time duration it takes before GFP expression (M2 activation)

Assay

• The cells will be grown until OD= 0.7.

• The RFP value will be monitered, although it is the GFP values that are more critical in this assay.

• OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].

• The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)

• The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)

See Glucose time delay for details