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Team:Imperial College London/Wetlab/Protocols/Autoinduction
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| Section | Assay | Overview and Aims | Promoter Characterisation | Secondary Carbon Source Diauxie Growth |
|
|---|---|---|---|---|---|
| Auto-Induction | Glucose Time Delay | * Characterise the tunable time duration it takes before GFP expression (M2 activation) |
Glucose time delay
Aims
- Characterise the tunable time duration it takes before GFP expression (M2 activation)
Assay
- The cells will be grown until OD= 0.7.
- The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
- OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
- The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
- The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
See Glucose time delay for details
