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Team:KULeuven/21 September 2009
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Revision as of 10:51, 24 September 2009 by JochemDeen (Talk | contribs)
Project progress
Progress of parts
[edit] Blue Light Receptor
- a new restriction digest on logA (1&2) was done.
- the re-enting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl. a restriction digest with EcoRI and PstI was performed and put on gel. the signals were good.
- a glycerol stock of ligY was made and some samples for sequencing were prepared.
- a motherplate of LogA en LikA were made and liquid cultures to make a glycerol stock.
- liquid cultures were also made for empty top10 cells, standard promotor and LikA (blp and GFP in pSB3K3).
[edit] Vanillin Production
- Plating of Pams&co
- Miniprepped electroporated PEFT
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| PEFT1 1 UV | 155,4 | 1,99 | |
| PEFT1 2 UV | 158,8 | 2,00 | |
| PEFT2 1 UV | 173,1 | 1,96 | |
| PEFT2 2 UV | 149,5 | 2,05 |
- All sizes where correct
- Made motherplate from PEFT1 UV
- Made motherplate from PAMS
- Made motherplate from EFT
[edit] Vanillin Receptor
- Again PstI din't cut overnight in both samples. A new restriction digest was done over one day because pstI did need maye more time to cut.
- Electroporation of virB showed good results so motherplates were made
