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Team:Aberdeen Scotland/WetLab/betagalactosidaseassay
From 2009.igem.org
University of Aberdeen - Pico Plumber
Contents |
Introduction
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Methods
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Results
The activity gained by in vivo reconstitution of the alpha and omega subunit inside the XL1-Blue{alpha/omegaLacZ} strain was said to be the maximum achievable and therefroe said to be 100% activity. All the other activities when given in percentage are compared to this maximum of activity.
Generally, an increased enzymatic activity is achieved with increasing incubation time which allows the two components, alpha and omega, to be built together. Measurements were taken at an incubation time of 2 minutes, 5 minutes, 10 minutes, 60 minutes, and 19 hours. However, the activity is higher for a mixing ration of 1:1 (alpha to omega) than for the other ratios (4:1 and 1:4).
Never the less, at an incubation time of 60 minutes all three different ratios gave a significant higher activity in comparison with the negative ontrols, but much less activity than the in vivo reconstitution of the alpha and omega subunit.
Discussion
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