Template:Team:KULeuven/24 September 2009/BlueLightReceptor

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  1. following the conclusions made on 23/09 a new experiment was set up:
  • liquid cultures were ented (and reented in the evening) and grown on two different temperatures: 37° and 25°.
  • from each of these growth conditions some of the cultures were put in 16° and the other at 25°. each of these groups were then again divided in a group that was lit with blue light and a group that wasn't.
  • this was done for our ligation of blp with GFP in three different vector (pSB3K3, pBR322 and pSB1A2)
  • an example of a sample name is: 37/16/NB/LigA, grown at 37°, put in 16°, not lit and in vector pSB1A2.
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