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Team:SDU-Denmark/Protocols
From 2009.igem.org
Lé Protocols
Primer PCR protocol - We used it for: Making a full RIP molecule from our oligos.
Purification protocol from gel - We used it for: Gel purification, over and over and over.
Purification protocol from solution - We used it for: PCR product purification.
Making cells competent - We used it for: Making competent cells for both transformation and electroporation.
Transformation - We used it for: Transforming plasmids (containing biobricks) into competent cells.
Electroporation - We used it for: Transforming plasmids (containing biobricks) into competent cells. Electroporation is our preferred technique, since it requires less plasmid volume and uses electricity!
Miniprep - We used it for: Purifying plasmids from inoculations of transformed bacteria.
How to make an agarose gel - We used it for: Making Agarose gels, for use in testing brick and plasmid sizes. Very central technique to controlling your lab work.
Restrictions - We used it for: Cutting out biobricks and preparing backbones for insertion of biobricks.
Ligations - We used it for: Pasting our biobricks into backbones.
