Imperial College London/Notebook/11 September 2009

Testing

Diauxic growth 11th Sep

Protocol considerations

• Using spectrophotometer at 600nm
• Blanks are all the relevant carbon sources, but subsequently realised that all blanks were roughly the same (within 0.03)
  • Therefore, subsequent readings= blank + 6 sample

• Overnight culture had OD of 1.64.
  • 800ul was put into each sample of 40ml~ 0.033 OD
  • ie same initial OD
• Grown in 6 separate 50ml falcon tubes and shaking in incubator at 28 degrees Celsius
• 1ml taken out at 1 hr intervals from the falcon tube and placed into spectrophotometer

Results

Download raw data

Conclusions

Suggestions/Improvements