Imperial College London/Notebook/2 October 2009

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Contents

Testing

OD calibration 4th run 2nd Oct

Protocol considerations

  • Cells were grown overnight in 10ml of M9 media
  • The cells were allowed to grow until a high OD in the late morning, then placed in cold room
  • OD of around 0.4 to 1.2 was made up, and subsequently diluted up to 10-4 to 10-6
  • 100ul of cells were inoculated into each plate
  • The OD of the overnight cells were measured again after 3 hours on ice to check if cell number changes during dilution process

Results

Waiting to be uploaded

Conclusions

  • After 3 hours on ice, the OD stays relatively constant
    • Shows that number of cells during OD measurement and during plating stay the same. Therefore, OD correlates with the number of cells that are plated.

Suggestions/Improvements

Waiting to be uploaded


Mr. Gene   Geneart   Clontech   Giant Microbes

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