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Team:Valencia/Parts/Characterization
From 2009.igem.org
General information
AEQ is a gene which encodes for aequorin, our luminiscent protein.
It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but if it binds to its cofactor coelenterazine, in presence of Ca2+, aequorin emits light.
Sequence
Aequorin sequence is (primer binding sites are underlined in green):
