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From 2009.igem.org
Design your own promotor
As one of the most important approaches to synthezise synthetic promotors we developed a database that predicts the position of conserved promotor binding sequences. We identified conserved sequences ourselves, analyzed the data and coded an easy to use interface that ise available for public use.
Mission 2009
Mammalian synthetic biology has huge potential, but it is in need of new standards and of systematic construction of comprehensive part libraries. Promoters are the fundamental elements of every synthetic biological system. We have developed and successfully applied two novel, in silico guided methods for the rational construction of synthetic promoters which respond only to predefined transcription factors.
Mission accomplished
We generated and characterized sets of constitutive and inducible promotors of different strength using our heartbeat-database prediction software and a new method developed in our lab. We combined a subset of these promotors with our targeted fluorescent protein tag and could thus show... Furthermore we characterized the eukaryotic standart
