August/14 August 2009

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miniprep

Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230] 

  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C)   29.5ng/μL　 1.56  0.91
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I)     7.5        1.88  2.79
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A)   11.5       1.92  2.65
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L)   28.6       1.81  2.46
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M)    10.1       1.96  2.60
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G)   14.0       1.99  2.47
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I)     15.4       1.86  3.29
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F)   17.9       1.99  2.60
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N)   11.2       1.88  2.32
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G)   19.5       1.97  2.34
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O)    11.0       1.60  1.74
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E)     5.5        1.46  2.03
  K204001 - 3    16.1       1.78  2.40
  K204001 - 8    14.4       1.77  2.38

transformation

to get new plasmid 

   sample No.
    ③　④　⑤　⑥

back to NOTES

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