Major results are shown in this section; there's a brief description of each and you can click through to find more detail on them. For minor results, please refer to our Notebook.

Module 1

Module 2

Module 3

Genome Deletion

• Restriction Enzyme testing in Dam+ve and Dam-ve Strains
  • An experiment investigating the effects of methylation on the amount of cleavage by varying concentrations of restriction enzymes.

Temporal Control

Diauxic Growth

Growth in the presence of Glucose only

Testing the effects of glucose concentration on cell growth: This experiment will be linked to diauxic growth.
Rationale: A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates.
Experiment: In a 96-well plate, initial concentrations of glucose ranging from 0.01% to 0.05% of the culture were studied. There are several possible effects that it could exert on cell growth and the hypotheses are stated below:
1.The lower the initial amount of glucose in the medium, the shorter the time taken for the population to reach saturation level.
2.The lower the initial amount of glucose in the medium, the lower the growth rate prior saturation level, but no delays on the time taken to reach saturation.
In addition, we also know that a lower amount of glucose means that the population will grow less and saturate at a smaller value.

Growth in the presence of Glucose + Xylose