Major results are shown in this section; there's a brief description of each and you can click through to find more detail on them. For minor results, please refer to our Notebook.

Module 1

Module 2

Module 3

Genome Deletion

• Restriction Enzyme testing in Dam+ve and Dam-ve Strains
  • An experiment investigating the effects of methylation on the amount of cleavage by varying concentrations of restriction enzymes.

Temporal Control

Diauxic Growth

Growth in the presence of Glucose only

Testing the effects of glucose concentration on cell growth: This experiment will be linked to diauxic growth.
Rationale: A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates.
Raw data 06/10 Media:II09_rawdata.xls

https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/Autoinduction/GluXy Growth in the presence of Glucose + Xylose

Testing the effects of different xylose concentrations on cell growth, for a fixed initial glucose concentration: This experiment will be linked to diauxic growth. Rationale: A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates. However, in the presence of a secondary carbon source, the cells enter a second exponential phase where they grow and saturate once again when the secondary source has been used up.