Team:UNIPV-Pavia/Methods Materials/Measurement

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A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.

The results of the experiments, grouped by argoments, are described here, if you need more informations about a single experiment, you can find the detailed protocol at [http://aimed11.unipv.it/iGEM2009 Download Protocols].

Studies:

ABSORBANCE:

• Absorbance and water dispensation
• Absorbance and volume
• Absorbance and dilutions in liquid growing medium

FLUORESCENCE:

• Fluorescence and water dispensation
• Fluorescence and dilutions in water
• Fluorescence and volume
• Fluorescence and dilutions in liquid growing medium
• Temporal evolution of fluorescence measurements
• Bleaching and autofluorescence

EVAPORATION:

• The "frame effect" on the plate