Team:UAB-Barcelona/Primersn

These were the primers we ordered for the nitrite:

• Pnir: EcoR1-Not-Xba— nirk – Spe

Reverse:

                S
    GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC    Annealing temperature = 62 ºC

Forward:

                 E      N       X
     CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC   Annealing temperature= 60 ºC

• nsrR: X – nsrR – S – N- P

Reverse:

                 X     RBS    Linker
     GTCAACGT TCTAGA TAAGGAGG AATATAA ATGAGACTGACGAATTACAGCGATTAC   Annealing temperature = 76 ºC

Forward:

                 P      N       S
     CAGTAGTA CTGCAG GCGGCCGC ACTAGT GCCAAGCTGACCTACAGTTTATTCC       Annealing temperature = 76 ºC