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Team:KULeuven/25 August 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
- Miniprepping the liquid cultures with
| Part | concentration (ng/μl) | 260/280 λ |
|---|---|---|
| (1) | 27,6 | 1,91 |
| (2) | 47,7 | 2,08 |
- Restriction digest of with EcoRI and PstI
- Gel electrophoresis and extraction of the RD of
--> failed. There was no signal at all
- New colonies were ented in liquid culture.
- Ligation C performed: (A) +
| Vector | insert |
| + | |
| 30ng -> 1,4μl | 40,8ng -> 2μl |
[edit] Vanillin Production
- We have colonies!!
- Lots of EF, enough of SAMS
- We made fluid cultures and let them grow overnight
[edit] Vanillin Receptor
- PCR mutagenesis from R on 427, PCR purification and gelelectrophoresis: no signal on the picture probably because enting didn't show satisfying results
- Ligation van X and Y in K14 at 16°C
- New enting of R202
- W has been ented on solid agar, so miniprep and nanodrop are scheduled for today
- Mutagenesis of W + PCR purification
