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Team:Uppsala-Sweden/Parts
From 2009.igem.org
Contents
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Parts
Here we list all the BioBricks we designed, constructed and used during the project.
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Regulatory Elements |
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Promoter |
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| BBa_K273019 | Regulatory | pPetE | 429 | |
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Alcohol Production |
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Butanol Parts, Intermediates and Devices |
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| BBa_K273006 | Coding | a-ketoisovalerate decarboxylase (kivd) codon optimized | 1650 | |
| BBa_K273008 | Intermediate | RBS - a-ketoisovalerate decarboxylase (kivd) codon optimized | 1668 | |
| BBa_K273005 | Coding | Alcohol Dehydrogenase 2 (Yadh2) | 1050 | |
| BBa_K273007 | Intermediate | RBS - Alcohol Dehydrogenase 2 (Yadh2) | 1068 | |
| BBa_K273009 | Intermediate | RBS - adh2(Y) - Terminator | 1205 | |
| BBa_K273012 | Intermediate | RBS - kivd - RBS - adh2 - Ter | 2881 | |
| BBa_K273011 | Composite | PLlac - RBS - kivd - RBS - adh2(Y) - Ter | 2944 | |
| BBa_K273012 | Composite | pPetE - RBS - kivd - RBS - adh2(Y) - Ter | 3318 | |
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Ethanol Parts and Devices |
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| BBa_K273000 | Coding | Pyruvate decarboxylase (pdc) | 1710 | |
| BBa_K273001 | Coding | Alcohol dehydrogenase (Zadh2) | 1155 | |
| BBa_K273004 | Intermediate | RBS - Pyruvate decarboxylase (pdc) | 1728 | |
| BBa_K273017 | Intermediate | RBS - Alcohol dehydrogenase (Zadh2) | 1173 | |
| BBa_K273018 | Intermediate | RBS - adh2(Z) - Ter | 1310 | |
| BBa_K273013 | Intermediate | RBS - pdc - RBS -adh2(Z) - Ter | 3046 | |
| BBa_K273056 | Composite | PLlac - RBS - pdc - RBS - adh2(Z) - Ter | 3109 | |
| BBa_K273057 | Composite | pPetE - RBS - pdc - RBS - adh2(Z) - Ter | 3483 | |
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Ethanol - S. cerevisae |
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| BBa_K273014 | Intermediate | RBS - pdc - RBS - adh2(Y) - Ter | 2941 | |
| BBa_K273015 | Composite | PLlac - RBS - pdc - RBS - adh2(Y) - Ter | 3004 | |
| BBa_K273016 | Composite | pPetE - RBS - pdc - RBS - adh2(Y) - Ter | 3378 | |
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Pyruvate Level Increasing Approaches |
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Pir Approach |
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| BBa_K273002 | Coding | Pirin like protein (pirA) | 702 | |
| BBa_K273003 | Coding | Pirin like protein (pirB) | 240 | |
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Antisense RNA Approach |
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| BBa_K273020 | RNA | E1A1 | 20 | |
| BBa_K273021 | RNA | E1A2 | 30 | |
| BBa_K273022 | RNA | E1A3 | 50 | |
| BBa_K273023 | RNA | E1A4 | 30 | |
| BBa_K273024 | RNA | E1B1 | 30 | |
| BBa_K273025 | RNA | E1B2 | 30 | |
| BBa_K273026 | RNA | E1B3 | 20 | |
| BBa_K273027 | RNA | E1B4 | 37 | |
| BBa_K273028 | RNA | E21 | 30 | |
| BBa_K273029 | RNA | E22 | 30 | |
| BBa_K273030 | RNA | E23 | 20 | |
| BBa_K273031 | RNA | E24 | 50 | |
| BBa_K273032 | Intermediate | E1A1 - Ter | 157 | |
| BBa_K273033 | Intermediate | E1A2 - Ter | 167 | |
| BBa_K273034 | Intermediate | E1A3 - Ter | 187 | |
| BBa_K273035 | Intermediate | E1A4 - Ter | 167 | |
| BBa_K273036 | Intermediate | E1B1 - Ter | 167 | |
| BBa_K273037 | Intermediate | E1B2 - Ter | 167 | |
| BBa_K273038 | Intermediate | E1B3 - Ter | 157 | |
| BBa_K273039 | Intermediate | E1B4 - Ter | 174 | |
| BBa_K273040 | Intermediate | E21 - Ter | 167 | |
| BBa_K273041 | Intermediate | E22 - Ter | 167 | |
| BBa_K273042 | Intermediate | E23 - Ter | 157 | |
| BBa_K273043 | Intermediate | E24 - Ter | 187 | |
| BBa_K273044 | Composite | pPetE - E1A1 - Ter | 594 | |
| BBa_K273045 | Composite | pPetE - E1A2 - Ter | 604 | |
| BBa_K273046 | Composite | pPetE - E1A3 - Ter | 624 | |
| BBa_K273047 | Composite | pPetE - E1A4 - Ter | 604 | |
| BBa_K273048 | Composite | pPetE - E1B1 - Ter | 604 | |
| BBa_K273049 | Composite | pPetE - E1B2 - Ter | 604 | |
| BBa_K273050 | Composite | pPetE - E1B3 - Ter | 594 | |
| BBa_K273051 | Composite | pPetE - E1B4 - Ter | 611 | |
| BBa_K273052 | Composite | pPetE - E21 - Ter | 604 | |
| BBa_K273053 | Composite | pPetE - E22 - Ter | 604 | |
| BBa_K273054 | Composite | pPetE - E23 - Ter | 594 | |
| BBa_K273055 | Composite | pPetE - E24 - Ter | 624 | |
