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Team:Chiba/Notebook/Calendar/2 September 2009
From 2009.igem.org
(Lab Work Start! |>3_September_2009)
Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)
- prior culture
medium:liquid culture medium antibiotic:Amp
We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.
Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)
Handwritten Lab Notebook
