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From 2009.igem.org

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August 8, 2009

1. BB1 transformants showed many colonies
   • plates (a plate full of colonies)
   • -> increasing heat shock to 90s increased efficiency (Note: 3x DNA used in this transformation)
   • Calvin mentioned he does heat shock for 120s
   • Calvin also suggested to increase tranformation time, ie. after adding DNA, hold on ice for an hour
2. DH-5alpha replates all grew on the plates, demonstrating LB can be re-autoclaved and poured as plates
   • there was no difference between transformants directly plated after adding LB+glucose or incubating for an hour
3. Miniprepped overnights (1+2) samle 1,2,4, and K

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