Team:UAB-Barcelona/Primersp

From 2009.igem.org

Encap raro.jpg Documento sin título

These were the primers we ordered for the phosphate:

• phoA: EcoR1-Not-Xba— phoA – Spe

Reverse: 

                 S
    5’ GCTCATA ACTAGT TTTATTTTCTCCATGTACAAATACATTAAAA 3’ Annealing temperature = 74 ºC

Forward: 

                    E      N       X
     5’ AGATTCA GAATTC GCGGCCGC TCTAGA GTTTCAACAGCTGTCATAAAGTTGTCAC  3’   Annealing temperature= 74 ºC
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