"
August/28 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1. Conducted miniprep followed by nanodrop concentration check on the following: 10-A, 10-B, 10-D, 10-E. Results of nanodrop check are as follows: Conc. 260/280 260/230 10-A ...) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | 1. Conducted miniprep followed by nanodrop concentration check on the following: 10-A, 10-B, 10-D, 10-E. Results of nanodrop check are as follows: | + | 1. Conducted miniprep followed by nanodrop concentration check on the following: 10-A, 10-B, 10-D, 10-E. <br> |
| + | Results of nanodrop check are as follows: | ||
Conc. 260/280 260/230 | Conc. 260/280 260/230 | ||
| - | 10-A 35.2 1.98 1.76 | + | 10-A 35.2 1.98 1.76 |
| - | 10-B 20.7 1.82 1.59 | + | |
| - | 10-D 37.3 1.90 1.84 | + | 10-B 20.7 1.82 1.59 |
| - | 10-E 23.7 1.94 1.76 | + | |
| + | 10-D 37.3 1.90 1.84 | ||
| + | |||
| + | 10-E 23.7 1.94 1.76 | ||
| - | 2. Conducted transformation for the following ligated parts: (12), (13), (14), (15), (16), (18), (19), (20), (21. Also BioBrick part 1-6I was transformed as a positive control. The plated-out cultures were incubated at 17:30pm. | + | 2. Conducted transformation for the following ligated parts: (12), (13), (14), (15), (16), (18), (19), (20), (21).<br> |
| + | Also BioBrick part 1-6I was transformed as a positive control.<br> The plated-out cultures were incubated at 17:30pm. | ||
3. Prepared 42 plates of amphicilin-resistance LA culture medium. | 3. Prepared 42 plates of amphicilin-resistance LA culture medium. | ||
| + | |||
| + | [https://2009.igem.org/Team:Osaka/NOTES bakc to NOTES] | ||
Latest revision as of 08:17, 12 October 2009
1. Conducted miniprep followed by nanodrop concentration check on the following: 10-A, 10-B, 10-D, 10-E.
Results of nanodrop check are as follows:
Conc. 260/280 260/230 10-A 35.2 1.98 1.76 10-B 20.7 1.82 1.59 10-D 37.3 1.90 1.84 10-E 23.7 1.94 1.76
2. Conducted transformation for the following ligated parts: (12), (13), (14), (15), (16), (18), (19), (20), (21).
Also BioBrick part 1-6I was transformed as a positive control.
The plated-out cultures were incubated at 17:30pm.
3. Prepared 42 plates of amphicilin-resistance LA culture medium.
