Illinois/7 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{UIUC09Temp}} {{Illinois}} {{IllinoisCalendar}} == July 7 == '''sRNA Library''' We ran two gels with all of our digestion products. Image:Digest1uiuc.jpg Lane 1 100bp ladder ...) |
|||
Line 1: | Line 1: | ||
{{UIUC09Temp}} | {{UIUC09Temp}} | ||
{{Illinois}} | {{Illinois}} | ||
+ | <html> | ||
+ | <div id="uicontentbox"> | ||
+ | </html> | ||
{{IllinoisCalendar}} | {{IllinoisCalendar}} | ||
Line 55: | Line 58: | ||
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | [https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | ||
- | + | <html> | |
+ | </div> | ||
+ | </html> | ||
{{IllinoisBottomNav}} | {{IllinoisBottomNav}} |
Latest revision as of 21:21, 20 July 2009
|
|
|
July 7
sRNA Library
We ran two gels with all of our digestion products.
Lane 1 100bp ladder
2 ftsZ 3 ftsZ 4 oppA 5 oppA 6 dppA 7 dppA 8 hns 9 hns 10 galK
Lane 1 100bp ladder
2 galK 3 dicF 4 dicF 5 gcvB 6 gcvB 7 rseX 8 rseX 9 ryhB 10 ryhB
The samples were split up into two lanes just in case the wells on the gels didn't overflow.
After the digestion gels, we performed a gel purification and used the nanodrop to check the DNA concentrations for each of the digestion products.
Concentrations: ng/microliter
ftsZ: 21.6 oppA: 14.1 dppA: 15.0 hns: 11.8 galK: 19.0 dicF: 23.0 gcvB: 21.3 rseX: 6.8 ryhB: 7.5