User contributions
From 2009.igem.org
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- 05:10, 15 October 2009 (diff | hist) Team:Washington/Notebook/NheI PstI
- 05:10, 15 October 2009 (diff | hist) Team:Washington/Notebook/colony PCR
- 05:10, 15 October 2009 (diff | hist) Team:Washington/Notebook/gene synthesis
- 05:09, 15 October 2009 (diff | hist) Team:Washington/Notebook/2mL purification
- 05:08, 15 October 2009 (diff | hist) Team:Washington/Notebook/Ni-column
- 05:08, 15 October 2009 (diff | hist) Team:Washington/Notebook/50mL purification
- 05:07, 15 October 2009 (diff | hist) Team:Washington/Notebook/Flow Cytometry
- 05:07, 15 October 2009 (diff | hist) Team:Washington/Notebook/Microscope
- 05:07, 15 October 2009 (diff | hist) Team:Washington/Notebook/protein gel
- 05:06, 15 October 2009 (diff | hist) Team:Washington/Notebook/NheI
- 04:59, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:52, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:17, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:17, 15 October 2009 (diff | hist) N Team:Washington/Notebook/NheI (New page: ==== Gene Assembly using the NheI site ==== #Start with first 23 coding nucleotides of gene, eg. 5'-atgcgtaaaggagaagaacttt...-3' #Replace the atg start codon with the XbaI site: 5'-TCTAGA-...)
- 04:15, 15 October 2009 (diff | hist) N Team:Washington/Notebook/NheI PstI (New page: ==== Gene Assembly using the NheI and PstI sites ==== #Start with first 23 coding nucleotides of gene, eg. 5'-atgcgtaaaggagaagaacttt...-3' #Replace the atg start codon with the XbaI site: ...)
- 04:14, 15 October 2009 (diff | hist) N Team:Washington/Notebook/colony PCR (New page: ==== Colony PCR ==== #Prepare one sterile 0.6mL tube with the following reaction mixture for each colony you intend to pick. ##5uL Qiagen Master Mix ##1uL 40uM VF2 ##1uL 40uM VR #Prepare o...)
- 04:13, 15 October 2009 (diff | hist) N Team:Washington/Notebook/gene synthesis (New page: ==== Gene Synthesis ==== #Generate Oligo's ##Go to: http://helixweb.nih.gov/dnaworks/ ##Set parameters ###Enter you job title and email ###Choose E.Coli Class II for codon frequency ###Se...)
- 04:10, 15 October 2009 (diff | hist) N Team:Washington/Notebook/2mL purification (New page: ==== Supernatant Protein Purification, 2mL ==== #Inoculate 50mL culture of TB with ~750uL overnight culture #Grow a 37c until OD600: 0.4 #Inoculate cells with IPTG so that the final concen...)
- 04:08, 15 October 2009 (diff | hist) N Team:Washington/Notebook/Ni-column (New page: ====Ni-column Set up==== #Transfer 2mL NiNTA beads (Quigen) to column #wash column with 10mL dH2O #Equillibrate column with 10mL running buffer (usually PBS) #To reuse column ##Wash with ...)
- 04:07, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:07, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:06, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:06, 15 October 2009 (diff | hist) N Team:Washington/Notebook/50mL purification (New page: ==== Supernatant Protein Purification, 50mL ==== #Inoculate 50mL culture of TB with ~750uL overnight culture #Grow a 37c until OD600: 0.4 #Inoculate cells with IPTG so that the final conce...)
- 04:03, 15 October 2009 (diff | hist) N Team:Washington/Notebook/Flow Cytometry (New page: ==== Flow Cytometry ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, p...)
- 04:00, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:57, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:55, 15 October 2009 (diff | hist) N Team:Washington/Notebook/Microscope (New page: ==== Microscope ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, place...)
- 03:54, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:53, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:52, 15 October 2009 (diff | hist) N Team:Washington/Notebook/protein gel (New page: ==== Protein Gel ==== # Set up overnights of parts 48-51 # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have all grown up uniformly start boilin...)
- 03:50, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:30, 15 October 2009 (diff | hist) Team:Washington/Notebook (→Gene Assemble using the NheI and PstI sites)
- 03:30, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:08, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 02:37, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 02:09, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 20:40, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 19:44, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 17:55, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 17:41, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 17:41, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 16:45, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 16:26, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:24, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:24, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:23, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:20, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:20, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:12, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:08, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:05, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:05, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:08, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:07, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:06, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:05, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:54, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:54, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:49, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:35, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:34, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:32, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:31, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:31, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:30, 8 October 2009 (diff | hist) N File:Josef.jpg (top)
- 04:23, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:19, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:48, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:33, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:31, 8 October 2009 (diff | hist) File:Target structure.png (uploaded a new version of "Image:Target structure.png") (top)
- 03:27, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:27, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:25, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:24, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:23, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:20, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:15, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 01:35, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 22:35, 7 October 2009 (diff | hist) Team:Washington/Accomplishments
- 19:37, 7 October 2009 (diff | hist) File:Main graphic.png (uploaded a new version of "Image:Main graphic.png")
- 17:11, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:10, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:04, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:03, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:00, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:00, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:59, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:57, 6 October 2009 (diff | hist) Team:Washington
- 21:49, 4 October 2009 (diff | hist) Team:Washington/Accomplishments
- 21:48, 4 October 2009 (diff | hist) File:Display structure.png (uploaded a new version of "Image:Display structure.png") (top)
- 21:39, 4 October 2009 (diff | hist) File:Secretion structure.png (uploaded a new version of "Image:Secretion structure.png") (top)
- 21:31, 4 October 2009 (diff | hist) N File:Secretion structure.png
- 21:30, 4 October 2009 (diff | hist) N File:Display structure.png
- 21:30, 4 October 2009 (diff | hist) N File:Target structure.png
- 18:30, 4 October 2009 (diff | hist) Team:Washington
- 18:29, 4 October 2009 (diff | hist) Team:Washington
- 18:23, 4 October 2009 (diff | hist) Team:Washington
- 18:17, 4 October 2009 (diff | hist) Team:Washington
- 18:15, 4 October 2009 (diff | hist) Team:Washington
- 18:11, 4 October 2009 (diff | hist) N File:Main graphic.png (Graphic for main slide)
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