User contributions
From 2009.igem.org
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- 20:10, 1 July 2009 (diff | hist) IBB Pune/10 June 2009
- 20:10, 1 July 2009 (diff | hist) N IBB Pune/10 June 2009 (New page: Isolated plasmid using Kudo's method. GOT A BAND!)
- 20:09, 1 July 2009 (diff | hist) N IBB Pune/9 June 2009 (New page: '''Amrita''':- got transformants in 5ng. Ordered primers for the competance factor and lysis genes.)
- 20:09, 1 July 2009 (diff | hist) IBB Pune/8 June 2009
- 20:08, 1 July 2009 (diff | hist) N IBB Pune/8 June 2009 (New page: Amrita tried Transformation By Rapid transit (tm) Sigma Kit. '''concs of pBR322 used''' 100pg, 500pg, 1ng,5ng)
- 20:08, 1 July 2009 (diff | hist) IBB Pune/7 June 2009
- 20:07, 1 July 2009 (diff | hist) IBB Pune/7 June 2009
- 20:07, 1 July 2009 (diff | hist) N IBB Pune/7 June 2009 (New page: ''Mandar Swetha and Sharvar''i- NO plasmid obtained from the OWW maxiprep protocol. Amrita inoculated culture for transformation.)
- 20:07, 1 July 2009 (diff | hist) N IBB Pune/6 June 2009 (New page: '''Sharvari and Sweth'''a - No plasmid obtained from Wt cells. decide to extract plasmid DNA from Dh5-a transformed with GFP. inoculated for overnight growth.)
- 20:06, 1 July 2009 (diff | hist) N IBB Pune/5 June 2009 (New page: Amrita:- Got transformation for 20 ng not for the 10 ng Sharvari and swetha tried the Kudo and olsen method for plasmid isolation and will run the gel tomoro.)
- 20:05, 1 July 2009 (diff | hist) IBB Pune/4 June 2009
- 20:04, 1 July 2009 (diff | hist) IBB Pune/4 June 2009
- 20:04, 1 July 2009 (diff | hist) IBB Pune/4 June 2009
- 20:04, 1 July 2009 (diff | hist) N IBB Pune/4 June 2009 (New page: Meeting with advisors Discussed about things to do first. To Standardize other protocols 1.Plasmid isolation 2.restriction digestion 3.Gel elution of desired fragment. 4.Ligation make ...)
- 20:03, 1 July 2009 (diff | hist) IBB Pune/3 June 2009
- 20:02, 1 July 2009 (diff | hist) IBB Pune/3 June 2009
- 20:02, 1 July 2009 (diff | hist) N IBB Pune/3 June 2009 (New page: Did a control transformation with GFP and pBR322 G1-5uL of plasmid prep G2- 1uL of ploasmid prep P1-20 ng of pBR322 P2-10ng of pBR322 see results tomoro WIKI pUT UP!!!)
- 20:01, 1 July 2009 (diff | hist) N IBB Pune/2 June 2009 (New page: Saw plates... no transformants!! :( meeting with the fellow team mates conclusions of the discussion: 1. Have to repeat transformation with Ca Cl2 2. Also try the TSS protocol mandar and...)
- 20:01, 1 July 2009 (diff | hist) N IBB Pune/1 June 2009 (New page: Tried to transform Dh5-a cells with a test biobrick (protocol used :CaCl2 method) biobrick used ---> constitutive promoter+RFP in amp resistant backbone address:- Plate 3-13M)
- 20:00, 1 July 2009 (diff | hist) N IBB Pune/29 May 2009 (New page: Step 2 of culture revival. The glycerol stocks were revived on LB plates and single colonies were picked from the plate and put into LB. Designed and ordered primers)
- 20:00, 1 July 2009 (diff | hist) N IBB Pune/28 May 2009 (New page: Culture revival from age old glycerol stocks from the -20 freezer. step one accomplished.)
- 19:58, 1 July 2009 (diff | hist) N IBB Pune/26 May 2009 (New page: This week is dedicated for all the reading work, ordering work and official work. Also some time will be utilized for discussion about the project ideas. And, Amrita and Rahul are a part...)
- 19:56, 1 July 2009 (diff | hist) N IBB Pune/15 May 2009 (New page: FINALLY the exams are over and the iGEM work is started. Advisor has recomended Amrita oak(4th year) and Rahul bhosale(Mtech) as tentative team members. Chinar goes home as his GRE date ...)
- 19:27, 1 July 2009 (diff | hist) Team:IBB Pune/Team
- 19:25, 1 July 2009 (diff | hist) N File:Samit.jpg (top)
- 19:21, 1 July 2009 (diff | hist) Team:IBB Pune/Team
- 19:20, 1 July 2009 (diff | hist) Team:IBB Pune/Team
- 19:19, 1 July 2009 (diff | hist) Team:IBB Pune/Team
- 20:26, 23 June 2009 (diff | hist) Team:IBB Pune/team (Removing all content from page) (top)
- 20:19, 23 June 2009 (diff | hist) Team:IBB Pune
- 05:08, 21 June 2009 (diff | hist) Team:IBB Pune/Project (→Project Details)
- 05:08, 21 June 2009 (diff | hist) Team:IBB Pune/Project
- 05:01, 21 June 2009 (diff | hist) N Team:IBB Pune/team (New page: our team consists of 7 undergraduate students and 2 advisors.)
- 04:45, 14 June 2009 (diff | hist) Team:IBB Pune
- 14:18, 8 June 2009 (diff | hist) Team:IBB Pune
- 04:59, 7 June 2009 (diff | hist) Team:IBB Pune
- 04:58, 7 June 2009 (diff | hist) Team:IBB Pune
- 04:58, 7 June 2009 (diff | hist) N Team:IBB Pune/style2 (New page: /******************************************************************************* DESIGN INFORMATION: describes color scheme, borders, fonts ********************************************...)
- 04:57, 7 June 2009 (diff | hist) Team:IBB Pune/style (top)
- 04:56, 7 June 2009 (diff | hist) Team:IBB Pune/style
- 04:54, 7 June 2009 (diff | hist) Team:IBB Pune
- 04:53, 7 June 2009 (diff | hist) N Team:IBB Pune/scrpt (New page: // SpryMenuBar.js - version 0.12 - Spry Pre-Release 1.6.1 // // Copyright (c) 2006. Adobe Systems Incorporated. // All rights reserved. // // Redistribution and use in source and binary fo...) (top)
- 04:51, 7 June 2009 (diff | hist) Team:IBB Pune
- 04:49, 7 June 2009 (diff | hist) Team:IBB Pune
- 04:45, 7 June 2009 (diff | hist) Team:IBB Pune/style
- 04:43, 7 June 2009 (diff | hist) Team:IBB Pune/style
- 04:41, 7 June 2009 (diff | hist) N File:SpryMenuBarRight.gif (top)
- 04:40, 7 June 2009 (diff | hist) N File:SpryMenuBarDown.gif (top)
- 04:39, 7 June 2009 (diff | hist) Team:IBB Pune/style
- 04:36, 7 June 2009 (diff | hist) N File:SpryMenuBarRightHover.gif (top)
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