Team:British Columbia

From 2009.igem.org

(Difference between revisions)
(E.coli Traffic Light: A flexible, modular, and transparent system for multi-level assessment of variable inputs.')
 
(36 intermediate revisions not shown)
Line 1: Line 1:
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
{{Template:UBCiGEM2009_menu_home}}
-
![[Image:IGEMFinalGoldDNA-logo.jpg|100x100px]]
+
=<font color="#00CC66">E.coli</font> <font color="#FFCC66">Traffic</font> <font color="#FF0000">Light</font>: <br> A ''flexible'', ''modular'', and ''transparent'' system for multi-level assessment of variable inputs.'=
-
!align="center"|[[Team:British_Columbia|Home]]
+
Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.
-
!align="center"|[[Team:British_Columbia/Team|The Team]]
+
-
!align="center"|[[Team:British_Columbia/Project|The Project]]
+
-
!align="center"|[[Team:British_Columbia/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:British_Columbia/Modeling|Modeling]]
+
-
!align="center"|[[Team:British_Columbia/Sponsor_Us|Sponsor Us!]]
+
-
!align="center"|[[Team:British_Columbia/Biosensor_Sensitivity|Biosensor Sensitivity Notebook]]
+
-
!align="center"|[[Team:British_Columbia/Biosensor_Logic_Gate|Biosensor Logic Gate Notebook]]
+
-
!align="center"|[[Team:British_Columbia/Lab_Notebook|General Lab Notebook]]
+
-
!align="center"|[[Team:British_Columbia/Bibliography|Bibliography]]
+
-
|}
+
-
{|align="justify"
+
Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").
-
|Formed in March 2009, this iGEM team is the first to enter from UBC, and hopes to do its alma mater proud.
+
 
-
|[[Image:iGEMFinalGoldDNA-logo.jpg|200x200px|right]]
+
 
-
|-
+
Click the colours of the traffic light to learn about its different subparts!
-
|
+
<!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] -->
-
We have selected two project tracks:
+
 
-
* To manipulate the sensitivity of a whole-cell biosensor
+
<html>
-
* To implement a logic gate-based decision system using said biosensor
+
 
-
The general scheme is to prototype the manipulations using an arabinose sensor coupled to a GFP reporter.
+
 
-
|}
+
<img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight">
-
[[Image:UBC2009-kayaking.jpg|centre|frame|Our Team]]
+
 
 +
<map name="trafficlight">
 +
<area shape="circle"
 +
      coords="774,121,54"
 +
      alt="Sensors: This links to the pBAD promoters that sense the arabinose."
 +
      title="Sensors: This links to the pBAD promoters that sense the arabinose."
 +
      href="/Team:British_Columbia/pBAD"
 +
/>
 +
 
 +
<area shape="circle"
 +
      coords="775,236,55"
 +
      alt="Lock and Key: this controls when the colors are produced"
 +
      title="Lock and Key: this controls when the colors are produced"
 +
      href="/Team:British_Columbia/LockandKey"
 +
/>
 +
 
 +
<area shape="circle"
 +
      coords="774,350,54"
 +
      alt="The Jammer: this mRNA sequence blocks the key from opening the lock."
 +
      title="The Jammer: this mRNA sequence blocks the key from opening the lock."
 +
      href="/Team:British_Columbia/Jammer"
 +
/>
 +
 
 +
</map>
 +
</html>
 +
 
 +
 
 +
 
 +
 
 +
<!--
 +
[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
 +
-->

Latest revision as of 03:58, 22 October 2009

E.coli Traffic Light:
A flexible, modular, and transparent system for multi-level assessment of variable inputs.'

Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.

Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").


Click the colours of the traffic light to learn about its different subparts!

Sensors: This links to the pBAD promoters that sense the arabinose. Lock and Key: this controls when the colors are produced The Jammer: this mRNA sequence blocks the key from opening the lock.