Team:Calgary/6 July 2009
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* Performed Antarctic Phosphotase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients. | * Performed Antarctic Phosphotase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients. | ||
+ | *Transformed J13002-LuxOD47E into TOP10 cells and plated on AC and C plates in 25 uL and 50 uL, left plates for overnight growth. | ||
+ | *Did a plasmid isolation of colony2X LuxOD47E overnight cultures. | ||
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Revision as of 23:30, 17 August 2009
UNIVERSITY OF CALGARY