Team:Calgary/Lab
From 2009.igem.org
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The major obstacle of this part of the project is ensuring that we have appropriate expression levels of the LuxP and LuxQ proteins. The creation of the engineered Δσ70 promoter driving the expression of LuxPQ will provide us with adequate levels of LuxP and LuxQ for efficient AI-2 signalling. In addition, this promoter will allow us to examine the robustness of AI-2 signalling with respect to LuxP and LuxQ levels. | The major obstacle of this part of the project is ensuring that we have appropriate expression levels of the LuxP and LuxQ proteins. The creation of the engineered Δσ70 promoter driving the expression of LuxPQ will provide us with adequate levels of LuxP and LuxQ for efficient AI-2 signalling. In addition, this promoter will allow us to examine the robustness of AI-2 signalling with respect to LuxP and LuxQ levels. | ||
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Revision as of 05:14, 24 July 2009
UNIVERSITY OF CALGARY