"
Team:Chiba/Notebook/Calendar/2 September 2009
From 2009.igem.org
(Difference between revisions)
(→Making Fluorescent Transfer Curve for the Practice of Lab Work) |
|||
| Line 6: | Line 6: | ||
<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html> | <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html> | ||
| - | == Making Fluorescent Transfer Curve for the Practice of Lab Work == | + | == Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)== |
*prior culture | *prior culture | ||
| Line 17: | Line 17: | ||
Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. | Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. | ||
(21:40-) | (21:40-) | ||
| - | |||
== Handwritten Lab Notebook == | == Handwritten Lab Notebook == | ||
[[Image:20090903-1.jpg|200px]] [[Image:Chiba_Labwork_02Sep09.jpg|200px]] | [[Image:20090903-1.jpg|200px]] [[Image:Chiba_Labwork_02Sep09.jpg|200px]] | ||
Latest revision as of 17:42, 23 September 2009
(Lab Work Start! |>3_September_2009)
Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)
- prior culture
medium:liquid culture medium antibiotic:Amp
We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.
Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)
Handwritten Lab Notebook
