Team:Chiba/Notebook/Calendar/7 September 2009

From 2009.igem.org

(Difference between revisions)
Line 5: Line 5:
([[Team:Chiba/Notebook/Calendar/6_September_2009|6_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]])
([[Team:Chiba/Notebook/Calendar/6_September_2009|6_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]])
-
 
+
== Measurement of Receiver's Responce by AHL concentration (1)==
*Making AHL water solution
*Making AHL water solution
We made 1 &mu;M, 10 &mu;M, 1 mM AHL water solution from 100 mM AHL water solution.
We made 1 &mu;M, 10 &mu;M, 1 mM AHL water solution from 100 mM AHL water solution.

Revision as of 17:49, 23 September 2009

>Go to the Notebook page

(6_September_2009 <|>8_September_2009)

Measurement of Receiver's Responce by AHL concentration (1)

  • Making AHL water solution

We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution.


  • Making plates

Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.


  • prior culture

We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) .


Lab Notebook

Chiba Labwork 07Sep09.jpg