Team:Chiba/Notebook/Calendar/8 September 2009

From 2009.igem.org

(Difference between revisions)
(→Measurement of Receiver's Responce by AHL concentration (1))
 
(31 intermediate revisions not shown)
Line 4: Line 4:
([[Team:Chiba/Notebook/Calendar/7_September_2009|7_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/9_September_2009|9_September_2009]])
([[Team:Chiba/Notebook/Calendar/7_September_2009|7_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/9_September_2009|9_September_2009]])
 +
 +
 +
== Measurement of Receiver's Responce by AHL concentration (1)==
 +
=== 13:55 Inoculation===
 +
We cultured a sample at 37 degrees Celsius.
 +
 +
 +
===16:15 Observation of GFP fluorescence ===
 +
We transferred cultured sample into plate.
 +
We observed samples every 30 min.
 +
Here are pictures of samples.
 +
 +
==== 16:15 (0) ====
 +
<gallery>
 +
Image:Chiba_0709_1615_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1615_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1615_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1615_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1615_1000_nM.jpg|1000 nM
 +
Image:Chiba_0709_1615_all.jpg‎ |All
 +
</gallery>
 +
 +
==== 16:45 (1) ====
 +
<gallery>
 +
Image:Chiba_0709_1645_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1645_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1645_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1645_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1645_1000_nM.jpg|1000 nM
 +
</gallery>
 +
 +
==== 17:15 (2)====
 +
<gallery>
 +
Image:Chiba_0709_1715_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1715_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1715_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1715_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1715_1000_nM.jpg|1000 nM
 +
Image:Chiba_0709_1715_all.jpg‎ |All
 +
</gallery>
 +
 +
==== 17:45 (3) ====
 +
<gallery>
 +
Image:Chiba_0709_1745_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1745_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1745_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1745_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1745_1000_nM.jpg|1000 nM
 +
Image:Chiba_0709_1745_all.jpg‎ |All
 +
</gallery>
 +
 +
==== 18:15 (4) ====
 +
<gallery>
 +
Image:Chiba_0709_1815_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1815_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1815_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1815_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1815_1000_nM.jpg|1000 nM
 +
Image:Chiba_0709_1815_all.jpg‎ |All
 +
</gallery>
 +
 +
==== 18:45 (5) ====
 +
<gallery>
 +
Image:Chiba_0709_1845_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1845_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1845_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1845_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1845_1000_nM.jpg|1000 nM
 +
Image:Chiba_0709_1845_all.jpg‎ |All
 +
</gallery>
 +
 +
==== 19:15 (6) ====
 +
<gallery>
 +
Image:Chiba_0709_1915_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1915_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1915_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1915_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1915_1000_nM.jpg|1000 nM
 +
Image:Chiba_0709_1915_all.jpg‎ |All
 +
</gallery>
 +
 +
==== 19:45 (7) ====
 +
<gallery>
 +
Image:Chiba_0709_1945_0_nM.jpg|0 nM
 +
Image:Chiba_0709_1945_1_nM.jpg|1 nM
 +
Image:Chiba_0709_1945_10_nM.jpg|10 nM
 +
Image:Chiba_0709_1945_100_nM.jpg|100 nM
 +
Image:Chiba_0709_1945_1000_nM.jpg|1000 nM
 +
</gallery>
 +
 +
== Check of Sender's AHL generation (1) Day 1 ==
 +
==== 20:45 start prior culture ====
 +
We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .
 +
 +
== Handwritten Lab Notebook ==
 +
[[Image:Chiba_Labwork_08Sep09.jpg|200px]]

Latest revision as of 18:22, 23 September 2009

>Go to the Notebook page

(7_September_2009 <|>9_September_2009)


Contents

Measurement of Receiver's Responce by AHL concentration (1)

13:55 Inoculation

We cultured a sample at 37 degrees Celsius.


16:15 Observation of GFP fluorescence

We transferred cultured sample into plate. We observed samples every 30 min. Here are pictures of samples.

16:15 (0)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

16:45 (1)

0 nM

1 nM

10 nM

100 nM

1000 nM

17:15 (2)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

17:45 (3)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

18:15 (4)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

18:45 (5)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

19:15 (6)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

19:45 (7)

0 nM

1 nM

10 nM

100 nM

1000 nM

Check of Sender's AHL generation (1) Day 1

20:45 start prior culture

We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .

Handwritten Lab Notebook

Chiba Labwork 08Sep09.jpg

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/8_September_2009"