Team:Chiba/Notebook/Calendar/8 September 2009

From 2009.igem.org

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(Measurement of Receiver's Responce by AHL concentration (1))
(Measurement of Receiver's Responce by AHL concentration (1))
 
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== Measurement of Receiver's Responce by AHL concentration (1)==
== Measurement of Receiver's Responce by AHL concentration (1)==
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*Inoculation
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=== 13:55 Inoculation===
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13:55
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We cultured a sample at 37 degrees Celsius.
We cultured a sample at 37 degrees Celsius.
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16:15
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===16:15 Observation of GFP fluorescence ===
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We transferred cultured sample into plate.
We transferred cultured sample into plate.
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=== Observation of GFP fluorescence ===
 
We observed samples every 30 min.
We observed samples every 30 min.
Here are pictures of samples.
Here are pictures of samples.
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Image:Chiba_0709_1945_1000_nM.jpg|1000 nM
Image:Chiba_0709_1945_1000_nM.jpg|1000 nM
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== Check of Sender's AHL generation (1) Day 1 ==
== Check of Sender's AHL generation (1) Day 1 ==

Latest revision as of 18:22, 23 September 2009

>Go to the Notebook page

(7_September_2009 <|>9_September_2009)


Contents

Measurement of Receiver's Responce by AHL concentration (1)

13:55 Inoculation

We cultured a sample at 37 degrees Celsius.


16:15 Observation of GFP fluorescence

We transferred cultured sample into plate. We observed samples every 30 min. Here are pictures of samples.

16:15 (0)

16:45 (1)

17:15 (2)

17:45 (3)

18:15 (4)

18:45 (5)

19:15 (6)

19:45 (7)

Check of Sender's AHL generation (1) Day 1

20:45 start prior culture

We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .

Handwritten Lab Notebook

Chiba Labwork 08Sep09.jpg