Team:Chiba/Notebook/Calendar/9 September 2009

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([[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/10_September_2009|10_September_2009]])
([[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/10_September_2009|10_September_2009]])
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== Check of Sender's AHL generation (1) ==
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=== 08:00 main culture ===
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==== 08:00 main culture ====
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:*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes  
:*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes  
:*WASH 3 times
:*WASH 3 times
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:*make 10 concentration varieties of solid media
:*make 10 concentration varieties of solid media
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== Lab Notebook ==
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== Handwritten Lab Notebook ==
[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]]
[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]]

Latest revision as of 18:25, 23 September 2009

>Go to the Notebook page

(8_September_2009 <|>10_September_2009)

Check of Sender's AHL generation (1)

08:00 main culture

  • divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
  • WASH 3 times
  • Add 12.5ml new culture
  • Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
  • Do same operation every 15min to 2nd~10th tubes
  • after all, mix the supernatant and solid media at 1:1
  • make 10 concentration varieties of solid media

Handwritten Lab Notebook

Chiba Labwork 09Sep09.jpg Chiba Labwork 09Sep09-2.jpg