Team:Gaston Day School/6 May 2009

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Transform pNICE ~ E. coli/dt>
50x competent cells on ice (Carolina Biol.)
200x cold 50mM CaCl2
10x 50ml pNICE (Lindow lab)
Ice 15' 8:11-->
Heat shock 42C 90"
Add 250x LB
1 hour 37C
Plate 100x, 100,x 200x, 200-250x
Overnight 37C

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