Team:Heidelberg/Parts

From 2009.igem.org

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__NOTOC__
__NOTOC__
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= Parts =
= Parts =
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== Measurement Kit ==
== Measurement Kit ==
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[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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== Core promoters ==
== Core promoters ==
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[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].
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== Promoters created by [[Team:Heidelberg/Project_Synthetic_promoters|RA-PCR]] ==
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== Promoters created by RA-PCR ==
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[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
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== HEARTBEAT Predicted promoters ==
== HEARTBEAT Predicted promoters ==
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[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]
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{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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|- style="background-color:#99cccc;"
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125  Part:BBa_K203125 ]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]
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|-
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|}
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== Natural promoters ==
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[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]
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{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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|- style="background-color:#99cccc;"
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | LDL receptor promoter (sterol regulated)  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]
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|-
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'''coming soon...'''
 
== Targeting Signals ==
== Targeting Signals ==
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[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]
Protein domains for targeting to sub-cellular compartments.
Protein domains for targeting to sub-cellular compartments.
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|- style="background-color:#9BDDFF;"  
|- style="background-color:#9BDDFF;"  
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
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|-
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" |  GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
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Latest revision as of 09:06, 4 July 2011

Parts

Measurement Kit

Main Article: Measurement

Name Part type Registry link
pSMB_MEASURE: Promoter Measurement plasmid (mammalian) Plasmid backbone Part:BBa_K203100
pSMB_REFERENCE Plasmid Part:BBa_K203099

Core promoters

Main Article: Core promoters

The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.

Name Part type Registry link
JeT Regulatory Part:BBa_K203112
JeT proximal-CMV core Regulatory Part:BBa_K203113

Promoters created by RA-PCR

Main article: Synthetic promoters

All contain the core promoter of Part:BBa_K203112 and were screened / measured in Part:BBa_K203100.

Name Part type Registry link
Constitutive promoter; 0.4 REU Regulatory Part:BBa_K203110
Constitutive promoter; 1 REU Regulatory Part:BBa_K203118
Constitutive promoter; 2 REU Regulatory Part:BBa_K203111
Constitutive promoter; 2.5 REU Regulatory Part:BBa_K203109
NF-κB regulated promoter Regulatory Part:BBa_K203119
pPARγ regulated promoter 1 Regulatory Part:BBa_K203114
pPARγ regulated promoter 2 Regulatory Part:BBa_K203115

HEARTBEAT Predicted promoters

Main article: HEARTBEAT Database

Name Part type Registry link
SREBP regulated promoter, predicted, tested HB8 Regulatory Part:BBa_K203125
SREBP regulated promoter, predicted, tested HB9 Regulatory Part:BBa_K203126

Natural promoters

Main article: Natural promoters

Name Part type Registry link
LDL receptor promoter (sterol regulated) Regulatory Part:BBa_K203105
HMG-CoA synthase promoter (sterol regulated) Regulatory Part:BBa_K203106
c-Jun promoter (growth factor regulated) Regulatory Part:BBa_K203107

Targeting Signals

Main article: Outlook and Summary

Protein domains for targeting to sub-cellular compartments.

Name Part type Registry link
Plasma membrane targeting (GPI anchor) Protein domain Part:BBa_K203000
Plasma membrane targeting (myrpalm) Protein domain Part:BBa_K203108
ER membrane targeting (C-terminal) Protein domain Part:BBa_K203101
ER membrane targeting (N-terminal) Protein domain Part:BBa_K203124
mCherry-myrpalm-polyA Composite Part:BBa_K203116
GFP-polyA Composite Part:BBa_K203117
Retrieved from "http://2009.igem.org/Team:Heidelberg/Parts"