Team:Heidelberg/Parts

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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{{Template_HD_3}}
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<html><body id="parts"></body></html>
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{|
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|-valign="top" border="0" style="margin-left: 2px;"
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|width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"|
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__NOTOC__
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= Parts =
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== Measurement Kit ==
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[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]
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{| class="wikitable centered" style="margin: 1em auto 1em auto"
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|- style="background-color:#99cccc;"  
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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This is a template page. READ THESE INSTRUCTIONS.
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|- style="background-color:#9BDDFF;"  
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</div>
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| style="font-weight:bold;" | pSMB_MEASURE: Promoter Measurement plasmid (mammalian) || Plasmid backbone || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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|- style="background-color:#9BDDFF;"  
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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|}
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your teams namespace. 
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</div>
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</div>
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</html>
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<!-- *** End of the alert box *** -->
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== Core promoters ==
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[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]
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The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].
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{|align="justify"
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{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|- style="background-color:#99cccc;"
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|[[Image:Example_logo.png|200px|right|frame]]
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | JeT  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]
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|}
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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== Promoters created by RA-PCR ==
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[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]
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All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
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{| class="wikitable centered" style="margin: 1em auto 1em auto"
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|- style="background-color:#99cccc;"
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | Constitutive promoter; 0.4 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | Constitutive promoter; 2 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | Constitutive promoter; 2.5 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | NF-&kappa;B regulated promoter || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | pPAR&gamma; regulated promoter 1 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | pPAR&gamma; regulated promoter 2 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]
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|align="center"|[[Team:Heidelberg | Team Example]]
 
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<!--- The Mission, Experiments --->
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== HEARTBEAT Predicted promoters ==
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[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]
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{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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|- style="background-color:#99cccc;"
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125  Part:BBa_K203125 ]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]
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|-
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|}
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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== Natural promoters ==
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!align="center"|[[Team:Heidelberg|Home]]
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!align="center"|[[Team:Heidelberg/Team|The Team]]
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[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]
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!align="center"|[[Team:Heidelberg/Project|The Project]]
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!align="center"|[[Team:Heidelberg/Parts|Parts Submitted to the Registry]]
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{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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!align="center"|[[Team:Heidelberg/Modeling|Modeling]]
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|- style="background-color:#99cccc;"  
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!align="center"|[[Team:Heidelberg/Notebook|Notebook]]
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"  
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| style="font-weight:bold;" | LDL receptor promoter (sterol regulated)  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]
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|-
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|}
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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== Targeting Signals ==
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===Note===
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[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
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Protein domains for targeting to sub-cellular compartments.
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{| class="wikitable centered"  style="margin: 1em auto 1em auto"
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|- style="background-color:#99cccc;"
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! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | Plasma membrane targeting (GPI anchor)  || Protein domain || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | Plasma membrane targeting (myrpalm) || Protein domain || [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | ER membrane targeting (C-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
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|-
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|- style="background-color:#9BDDFF;"
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| style="font-weight:bold;" | GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
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Latest revision as of 09:06, 4 July 2011

Parts

Measurement Kit

Main Article: Measurement

Name Part type Registry link
pSMB_MEASURE: Promoter Measurement plasmid (mammalian) Plasmid backbone Part:BBa_K203100
pSMB_REFERENCE Plasmid Part:BBa_K203099

Core promoters

Main Article: Core promoters

The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.

Name Part type Registry link
JeT Regulatory Part:BBa_K203112
JeT proximal-CMV core Regulatory Part:BBa_K203113

Promoters created by RA-PCR

Main article: Synthetic promoters

All contain the core promoter of Part:BBa_K203112 and were screened / measured in Part:BBa_K203100.

Name Part type Registry link
Constitutive promoter; 0.4 REU Regulatory Part:BBa_K203110
Constitutive promoter; 1 REU Regulatory Part:BBa_K203118
Constitutive promoter; 2 REU Regulatory Part:BBa_K203111
Constitutive promoter; 2.5 REU Regulatory Part:BBa_K203109
NF-κB regulated promoter Regulatory Part:BBa_K203119
pPARγ regulated promoter 1 Regulatory Part:BBa_K203114
pPARγ regulated promoter 2 Regulatory Part:BBa_K203115

HEARTBEAT Predicted promoters

Main article: HEARTBEAT Database

Name Part type Registry link
SREBP regulated promoter, predicted, tested HB8 Regulatory Part:BBa_K203125
SREBP regulated promoter, predicted, tested HB9 Regulatory Part:BBa_K203126

Natural promoters

Main article: Natural promoters

Name Part type Registry link
LDL receptor promoter (sterol regulated) Regulatory Part:BBa_K203105
HMG-CoA synthase promoter (sterol regulated) Regulatory Part:BBa_K203106
c-Jun promoter (growth factor regulated) Regulatory Part:BBa_K203107

Targeting Signals

Main article: Outlook and Summary

Protein domains for targeting to sub-cellular compartments.

Name Part type Registry link
Plasma membrane targeting (GPI anchor) Protein domain Part:BBa_K203000
Plasma membrane targeting (myrpalm) Protein domain Part:BBa_K203108
ER membrane targeting (C-terminal) Protein domain Part:BBa_K203101
ER membrane targeting (N-terminal) Protein domain Part:BBa_K203124
mCherry-myrpalm-polyA Composite Part:BBa_K203116
GFP-polyA Composite Part:BBa_K203117
Retrieved from "http://2009.igem.org/Team:Heidelberg/Parts"